Upload your assembled FASTA genome files.

Choose your tools:



Phylogenetic analysis.

MSA Trimming

Automated removal of spurious sequences or poorly aligned regions

Multiple sequence alignment (MSA)

Produces biologically meaningful multiple sequence alignments

Genome completeness

Genome completeness

Genome completeness and identification of orthologous genes.

Average nucleotide identity

Average nucleotide identity

Comparing average nucleotide identity.

Gene comparison

Gene comparison

Compare all previously predicted protein-coding genes against eachother.

UID Name FASTA files Submission Date Start date End date Mode Status
9237305b CrBVI.fa 2021/11/08 18:02 2021/11/08 18:03 2021/11/08 20:01 Annotation Finished
60b5f985 Branchiostoma floridae pipeline 2021/12/07 19:10 2021/12/07 19:11 2021/23/07 00:31 Annotation Failed
ec6d1246 ptk.fa 2021/07/07 14:11 2021/07/07 15:04 2021/07/07 15:05 Annotation Finished

The Modular Open-Source Genome Annotator (MOSGA) is a pipeline that easily creates draft genome annotation by a graphical user interface. It combines several specific prediction tools and generates a submission-ready annotation file.

The source code is freely available on Gitlab.com. We recommend building a new docker container from the available Dockerfile in the linked Gitlab repository. MOSGA is written modular and allows easy integration of new prediction tools or even including whole third-party pipelines.

For any questions or comments, please contact us: roman.martin@uni-marburg.de. We are happy to receive new suggestions or even merge requests for a pipeline extension. To provide an overview of the operation principle, we recommend reading our Gitlab wiki page.

We are providing an example data set of the draft genome annotation of Cafeteria roenbergensis BVI strain. Initially, we used an early version of MOSGA to annotate this genome (Hackl et al., 2020). Hackl, T., Martin, R., Barenhoff, K. et al. Four high-quality draft genome assemblies of the marine heterotrophic nanoflagellate Cafeteria roenbergensis. Sci Data 7, 29 (2020).

We provide two examples for the comparative genomics workflow: The Saccharomyces species phylogenetics and the Saccharomyces gene comparison. An exemplary annotation job for the organelle scanner based on the Nannochloropsis oceanica genome is here available.

Please take care about the licenses of the selected tools.

Whenever you use MOSGA please cite us:
Roman Martin orcid, Thomas Hackl orcid, Georges Hattab orcid, Matthias Fischer orcid, Dominik Heider orcid (2020). MOSGA: Modular Open-Source Genome Annotator. Bioinformatics. 36(22-23). 5514–5515. doi: 10.1093/bioinformatics/btaa1003.

Roman Martin orcid, Hagen Dreßler orcid, Georges Hattab orcid, Thomas Hackl orcid, Matthias Fischer orcid, Dominik Heider orcid (2021). MOSGA 2: Comparative genomics and validation tools. bioRxiv 2021.07.29.454382. doi: 10.1101/2021.07.29.454382.

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