The Modular Open-Source Genome Annotator (MOSGA)
is a pipeline that easily creates draft genome annotation by a graphical user interface.
It combines several specific prediction tools and generates a submission-ready annotation file.
The source code is freely available on Gitlab.com. We recommend building a new docker container from the available Dockerfile in the linked Gitlab repository. MOSGA is written modular and allows easy integration of new prediction tools or even including whole third-party pipelines.
For any questions or comments, please contact us: email@example.com. We are happy to receive new suggestions or even merge requests for a pipeline extension. To provide an overview of the operation principle, we recommend reading our Gitlab wiki page.
We are providing an example data set of the draft genome annotation of Cafeteria roenbergensis BVI strain. Initially, we used an early version of MOSGA to annotate this genome (Hackl et al., 2020). Hackl, T., Martin, R., Barenhoff, K. et al. Four high-quality draft genome assemblies of the marine heterotrophic nanoflagellate Cafeteria roenbergensis. Sci Data 7, 29 (2020).
We provide two examples for the comparative genomics workflow: The Saccharomyces species phylogenetics and the Saccharomyces gene comparison. An exemplary annotation job for the organelle scanner based on the Nannochloropsis oceanica genome is here available.
Please take care about the licenses of the selected tools.
Whenever you use MOSGA please cite us:
Roman Martin , Thomas Hackl , Georges Hattab , Matthias Fischer , Dominik Heider (2020). MOSGA: Modular Open-Source Genome Annotator. Bioinformatics. 36(22-23). 5514–5515. doi: 10.1093/bioinformatics/btaa1003.
Roman Martin , Hagen Dreßler , Georges Hattab , Thomas Hackl , Matthias Fischer , Dominik Heider (2021). MOSGA 2: Comparative genomics and validation tools. bioRxiv 2021.07.29.454382. doi: 10.1101/2021.07.29.454382.